Nanostructured conducting polymer based reagentless capacitive immunosensor
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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Title |
Nanostructured conducting polymer based reagentless
capacitive immunosensor
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Creator |
Bandodkar, Amay Jairaj
Dhand, Chetna Arya, Sunil K. Pandey, M. K. Malhotra, Bansi D. |
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Subject |
Biochemistry & Molecular Biology
Electrochemistry Engineering |
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Description |
Nanostructured polyaniline (PANI) film electrophoretically
fabricated onto indium-tin-oxide (ITO) coated glass plate has been utilized for development of an immunosensor
based on capacitance change of a parallel plate capacitor (PPC) by covalently immobilizing anti-human IgG (Anti-HIgG) using N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. These fabricated PANI/ITO and Anti-HIgG/PANI/ITO plates have been characterized using scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry and Fourier transform infra-red studies. The capacitance measurements indicate that dielectric medium of this biologically modified
PPC (Anti-HIgG/PANI/ITO) is sensitive to HIgG in 5−
5×105 ng mL−1 range and has lower detection limit of
1.87 ng mL−1. The observed results reveal that this Anti-
HIgG modified PPC can be used as a robust, easy-to-use,
reagentless, sensitive and selective immunosensor for estimation of human IgG.
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Publisher |
Springer Verlag
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Date |
2009-10-01
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://npl.csircentral.net/253/1/218.pdf
Bandodkar, Amay Jairaj and Dhand, Chetna and Arya, Sunil K. and Pandey, M. K. and Malhotra, Bansi D. (2009) Nanostructured conducting polymer based reagentless capacitive immunosensor. Biomedical Microdevices , 12 (1). pp. 63-70. ISSN 1572-8781 |
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Relation |
http://npl.csircentral.net/253/
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