Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection
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| Creator |
Kaushik, Ajeet
Solanki, Pratima R. Kaneto, Keiichi Kim, C. G. Ahmad, Sharif Malhotra, Bansi D. |
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| Subject |
Chemistry
Electrochemistry |
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| Description |
A nanostructured iron oxide (NanoFe(3)O(4), particle size ca. 25 nm and roughness ca. 2:1 nm) film deposited onto a hydrolyzed indium-tin-oxide (ITO) coated glass plate has been used to immobilize cholesterol oxidase (ChOx) to fabricate an impedimetric cholesterol sensor. Electrochemical studies reveal that surface charged Fe(3)O(4) nanoparticles provide better conformation for ChOx loading resulting in enhanced electron transfer between ChOx and the electrode. Impedimetric response studies of the ChOx/NanoFe(3)O(4)/ITO bioelectrode exhibit improved linearity (2.5-400 mg/dL), low detection limit (0.25 mg/dL), fast response time (25 s), high sensitivity (86 Omega/mg dL(-1)/cm(-2)) and a low value of the Michaelis-Menten constant (K(m) 0.8 mg/dL) with a regression coefficient of 0.997.
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| Publisher |
Wiley-VCH Verlag Berlin
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| Date |
2009-11-10
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/254/1/219.pdf
Kaushik, Ajeet and Solanki, Pratima R. and Kaneto, Keiichi and Kim, C. G. and Ahmad, Sharif and Malhotra, Bansi D. (2009) Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection. Electroanalysis , 22 (10). 1045 -1055. ISSN 1521-4109 |
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| Relation |
http://npl.csircentral.net/254/
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