Nanostructured zinc oxide platform for mycotoxin detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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Title |
Nanostructured zinc oxide platform for mycotoxin detection
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Creator |
Ansari, Anees A.
Kaushik, Ajeet Solanki, Pratima R. Malhotra, B. D. |
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Subject |
Biochemistry & Molecular Biology
Biophysics Electrochemistry |
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Description |
Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium–tin–oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as ~5.0 nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006–0.01 nM/dm3, detection limit of 0.006 nM/dm3, response time as 25 s and sensitivity of 189 Ω/nM/dm3cm− 2 with a regression coefficient of 0.997.
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Publisher |
Elsevier
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Date |
2009-06-25
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://npl.csircentral.net/256/1/221.pdf
Ansari, Anees A. and Kaushik, Ajeet and Solanki, Pratima R. and Malhotra, B. D. (2009) Nanostructured zinc oxide platform for mycotoxin detection. Bioelectrochemistry , 77 (2). pp. 75-81. ISSN 1878-562X |
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Relation |
http://dx.doi.org/10.1016/j.bioelechem.2009.06.014
http://npl.csircentral.net/256/ |
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