Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection
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| Creator |
Cao, Shunsheng
Mishra, Rajeev Pilla, Srikanth Tripathi, Swapnil Pandey, Manoj K. Shah, Gopit Mishra, Ajay K. Prabaharan, Mani Mishra, Shivani B. Xin, Jin Pandey, R. R. Wu, Weiwei Pandey, Avinash C. Tiwari, Ashutosh |
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| Subject |
Chemistry
Polymer Science |
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| Description |
A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid, Au-MPA NPs). The prepared CHIT/Au-MPA/ITO and the ssODNs/CHIT/Au-MPA/ITO electrodes were characterized with FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). TEM micrograph of the composite electrode showed that Au-MPA NPs had the diameter ranging from 4 to 16 nm. The ssODNs/CHIT/Au-MPA/ITO electrode showed a linear increase in the amperometric current with an increasing concentration of the single-stranded target complementary ODNs (cODNs) within the range of 0.03–325 fM. The ssODNs/CHIT/Au-MPA/ITO electrode exhibited a sensitivity of 0.572 μA/fM with a response time of 12 s. The ssODNs/CHIT/Au-MPA/ITO electrode had a shelf life of ∼182 days, when stored at 4 °C.
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| Publisher |
Elsevier
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| Date |
2010-04-21
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/263/1/228.pdf
Cao, Shunsheng and Mishra, Rajeev and Pilla, Srikanth and Tripathi, Swapnil and Pandey, Manoj K. and Shah, Gopit and Mishra, Ajay K. and Prabaharan, Mani and Mishra, Shivani B. and Xin, Jin and Pandey, R. R. and Wu, Weiwei and Pandey, Avinash C. and Tiwari, Ashutosh (2010) Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection. Carbohydrate Polymers , 82 (1). pp. 189-194. ISSN 0144-8617 |
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| Relation |
http://dx.doi.org/10.1016/j.carbpol.2010.04.051
http://npl.csircentral.net/263/ |
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