Peptide Nucleic Acid Immobilized Biocompatible Silane Nanocomposite Platform for Mycobacterium tuberculosis Detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Peptide Nucleic Acid Immobilized Biocompatible Silane Nanocomposite Platform for Mycobacterium tuberculosis Detection
|
|
| Creator |
Prabhakar, Nirmal
Solanki, Pratima R. Kaushik, Ajeet Pandey, M. K. Malhotra, B. D. |
|
| Subject |
Chemistry
Electrochemistry |
|
| Description |
We have prepared nanocomposite films comprising of 3-glycidoxypropyltrimethoxysilane (GOPS) and iron-oxide (Fe3O4) onto indium-tin-oxide (ITO) glass plate for covalent immobilization of 21-mer peptide nucleic acid (PNA). These films have been characterized using contact angle, atomic force microscopy (AFM), electrochemical techniques. The electrochemical response of the GOPS/ITO and Fe3O4-GOPS/ITO electrodes has been investigated by hybridization with complementary, non-complementary and one-base mismatch using methylene blue as electrochemical indicator. The PNA/Fe3O4-GOPS/ITO bioelectrode exhibits improved specificity and detection limit (0.1 fM) as compared to that of the PNA-GOPS/ITO bioelectrode (0.1 pM). This PNA/Fe3O4-GOPS/ITO electrode can be utilized for detection of hybridization with the complementary sequence in sonicated M. tuberculosis genomic DNA within 90 s of hybridization time.
|
|
| Publisher |
Wiley-VCH Verlag Berlin
|
|
| Date |
2010-11
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://npl.csircentral.net/274/1/239.pdf
Prabhakar, Nirmal and Solanki, Pratima R. and Kaushik, Ajeet and Pandey, M. K. and Malhotra, B. D. (2010) Peptide Nucleic Acid Immobilized Biocompatible Silane Nanocomposite Platform for Mycobacterium tuberculosis Detection. Electroanalysis , 22 (22). pp. 2672-2682. ISSN 1521-4109 |
|
| Relation |
http://npl.csircentral.net/274/
|
|