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Polyaniline/carbon nanotubes platform for sexually transmitted disease detection

IR@NPL: CSIR-National Physical Laboratory, New Delhi


Field	Value

Title	Polyaniline/carbon nanotubes platform for sexually transmitted disease detection

Creator	Singh, Renu Dhand, Chetna Sumana, Gajjala Verma, Rachna Sood, Seema Gupta, Rajinder Kumar Malhotra, Bansi Dhar

Subject	Biochemistry & Molecular Biology Biophysics

Description	Polyaniline/carbon nanotubes composite (PANI-CNT) electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate has been utilized for Neisseria gonorrhoeae detection by immobilizing 5′-amino-labeled Neisseria gonorrhoeae probe (aDNA) using glutaraldehyde as a cross-linker. PANI-CNT/ITO and aDNA-Glu-PANI-CNT/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect N. gonorrhoeae using methylene blue (MB) as redox indicator with response time of 60 s and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1 × 10−6 M to 1 × 10−17 M with detection limit of 1.2 × 10−17 M. Further, this bioelectrode (aDNA-Glu-PANI-CNT/ITO) exhibits specificity toward N. gonorrhoeae species and shows negative response with non-Neisseria gonorrhoeaeNeisseria species (NgNS) and other gram negative bacteria (GNB).

Publisher	John Wiley and Sons

Date	2010-01-25

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://npl.csircentral.net/279/1/244.pdf Singh, Renu and Dhand, Chetna and Sumana, Gajjala and Verma, Rachna and Sood, Seema and Gupta, Rajinder Kumar and Malhotra, Bansi Dhar (2010) Polyaniline/carbon nanotubes platform for sexually transmitted disease detection. Journal of Molecular Recognition , 23 (5). pp. 472-479. ISSN 1099-1352

Relation	http://npl.csircentral.net/279/