Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection
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| Creator |
Das, Maumita
Sumana, Gajjala R. , Nagarajan Malhotra, B. D. |
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| Subject |
Physics
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| Description |
Nanostructured zirconium oxide (ZrO2) film (particle size ∼ 35 nm), electrochemically deposited onto gold(Au) surface, has been used to immobilize 21-mer oligonucleotide probe (ssDNA) specific to Mycobacterium tuberculosis by utilizing affinity between oxygen atom of phosphoric group and zirconium to fabricate DNA biosensor. This DNA-ZrO2/Au bioelectrode, characterized using x-ray diffraction, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy techniques, can be used for early and rapid diagnosis of M. tuberculosis with detection limit of 0.065 ng/μL within 60s.
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| Publisher |
American Institute of Physics
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/395/1/354.pdf
Das, Maumita and Sumana, Gajjala and R. , Nagarajan and Malhotra, B. D. (2010) Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection. Applied Physics Letters, 96 (13). 133703-1-133703-3. ISSN 1077-3118 |
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| Relation |
http://dx.doi.org/10.1063/1.3293447
http://npl.csircentral.net/395/ |
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