A nanostructured cerium oxide film-based immunosensor for mycotoxin detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
A nanostructured cerium oxide film-based immunosensor for mycotoxin detection
|
|
| Creator |
Kaushik, Ajeet
Solanki, Pratima Rathee Ansari, Anees Ahmad Ahmad, Sharif Malhotra, Bansi Dhar |
|
| Subject |
Materials Science
Physics |
|
| Description |
Rabbit-immunoglobulin antibodies (r-IgGs) and bovine serum albumin (BSA) have been immobilized onto sol–gel-derived nanostructured cerium oxide (nanoCeO2) film fabricated onto an indium–tin-oxide (ITO) coated glass plate to detect ochratoxin-A (OTA). Broad reflection planes obtained in x-ray diffraction (XRD) patterns reveal the formation of CeO2 nanostructures. Electrochemical studies reveal that nanoCeO2 particles provide an increased electroactive surface area for loading of r-IgGs with desired orientation, resulting in enhanced electron communication between r-IgGs and electrode. BSA/r-IgGs/nano CeO2/ITO immunoelectrode exhibits improved characteristics such as linear range (0.5–6 ng dl−1), low detection limit (0.25 ng dl−1), fast response time (30 s) and high sensitivity (1.27 µA ng−1 dl−1 cm−2). The high value of the association constant (Ka, 0.9 × 1011 l mol−1) indicates the high affinity of the BSA/r-IgGs/nanoCeO2/ITO immunoelectrode to OTA.
|
|
| Publisher |
Institute of Physics
|
|
| Date |
2008-11-26
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://npl.csircentral.net/576/1/179.pdf
Kaushik, Ajeet and Solanki, Pratima Rathee and Ansari, Anees Ahmad and Ahmad, Sharif and Malhotra, Bansi Dhar (2008) A nanostructured cerium oxide film-based immunosensor for mycotoxin detection. Nanotechnology , 20 (5). 055105. ISSN 1361-6528 |
|
| Relation |
http://npl.csircentral.net/576/
|
|