A self assembled monolayer based microfluidic sensor for urea detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
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| Title |
A self assembled monolayer based microfluidic sensor for urea detection
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| Creator |
Srivastava, Saurabh
Solanki, Pratima R. Kaushik, Ajeet Ali, Md. Azahar Srivastava, Anchal Malhotra, B. D. |
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| Subject |
Chemistry
Materials Science Applied Physics/Condensed Matter Nanoscience/ Nanotechnology Physics |
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| Description |
Urease (Urs) and glutamate dehydrogenase (GLDH) have been covalently co-immobilized onto a self-assembled monolayer (SAM) comprising of 10-carboxy-1-decanthiol (CDT) via EDC–NHS chemistry deposited onto one of the two patterned gold (Au) electrodes for estimation of urea using poly(dimethylsiloxane) based microfluidic channels (2 cm × 200 μm × 200 μm). The CDT/Au and Urs-GLDH/CDT/Au electrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, contact angle (CA), atomic force microscopy (AFM) and electrochemical cyclic voltammetry (CV) techniques. The electrochemical response measurement of a Urs-GLDH/CDT/Au bioelectrode obtained as a function of urea concentration using CV yield linearity as 10 to 100 mg dl−1, detection limit as 9 mg dl−1 and high sensitivity as 7.5 μA mM−1 cm−2.
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| Publisher |
Royal Society of Chemistry
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| Date |
2011-04-15
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/778/1/7.pdf
Srivastava, Saurabh and Solanki, Pratima R. and Kaushik, Ajeet and Ali, Md. Azahar and Srivastava, Anchal and Malhotra, B. D. (2011) A self assembled monolayer based microfluidic sensor for urea detection. Nanoscale , 3 (7). pp. 2971-2977. ISSN 2040-3372 |
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| Relation |
http://dx.doi.org/10.1039/C1NR10240K
http://npl.csircentral.net/778/ |
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