Use of the Cryptogein Gene to Stimulate the Accumulation of Bacopa Saponins in Transgenic Bacopa Monnieri Plants
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Use of the Cryptogein Gene to Stimulate the Accumulation of Bacopa Saponins in Transgenic Bacopa Monnieri Plants
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| Creator |
Majumdar, Sukanya
Garai , Saraswati Jha, Sumita |
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| Subject |
Chemistry
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| Description |
Genetic transformation of the Indian medicinal
plant, Bacopa monnieri, using a gene encoding cryptogein,
a proteinaceous elicitor, via Ri and Ti plasmids, were
established and induced bioproduction of bacopa saponins
in crypt-transgenic plants were obtained. Transformed
roots obtained with A. rhizogenes strain LBA 9402 crypt on
selection medium containing kanamycin (100 mg l-1) dedifferentiated
forming callus and redifferentiated to roots
which, spontaneously showed shoot bud induction. Ri
crypt-transformed plants thus obtained showed integration
and expression of rol genes as well as crypt gene. Ti crypttransformed
B. monnieri plants were established following
transformation with disarmed A. tumefaciens strain harboring
crypt. Transgenic plants showed significant
enhancement in growth and bacopa saponin content.
Bacopasaponin D (1.4–1.69 %) was maximally enhanced
in transgenic plants containing crypt. In comparison to
Ri-transformed plants, Ri crypt-transformed plants showed
significantly (p B 0.05) enhanced accumulation of bacoside
A3, bacopasaponin D, bacopaside II, bacopaside III
and bacopaside V. Produced transgenic lines can be used
for further research on elicitation in crypt-transgenic plants
as well as for large scale production of saponins.
Key message The cryptogein gene, which encodes a proteinaceous
elicitor is associated with increase in secondary
metabolite accumulation—either alone or in addition to the
increases associated with transformation by A. rhizogenes
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| Publisher |
Springer Verlag
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1720/1/PLANT_CELL_REPORTS___31(10)_1899%2D1909;2012[35].pdf
Majumdar, Sukanya and Garai , Saraswati and Jha, Sumita (2012) Use of the Cryptogein Gene to Stimulate the Accumulation of Bacopa Saponins in Transgenic Bacopa Monnieri Plants. Plant Cell Reports, 31 (10). pp. 1899-1909. |
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| Relation |
http://dx.doi.org/10.1007/s00299-012-1303-3
http://www.eprints.iicb.res.in/1720/ |
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