A study on the effects of some laboratory-derived genetic mutations on biofilm formation by Listeria monocytogenes
IR@NIO: CSIR-National Institute Of Oceanography, Goa
View Archive Info| Field | Value | |
| Creator |
Kumar, S.
Parvathi, A. George, J. Krohne, G. Karunasagar, Indrani Karunasagar, Iddya |
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| Date |
2009-03-30T10:16:37Z
2009-03-30T10:16:37Z 2009 |
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| Identifier |
World Journal of Microbiology and Biotechnology, vol.25(3); 527-531
http://drs.nio.org/drs/handle/2264/2604 |
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| Description |
Biofilms formed by the human pathogen Listeria monocytogenes in food-processing environments can be a potential source of contamination. In this study, we investigated the ability of L. monocytogenes wild type and its laboratory-derived isogenic mutants in cwhA, prfA, agrA, flaA, degU, ami and sigB to adhere to and form biofilms on abiotic surfaces. The results suggest that inactivation of the two component regulatory system degU completely abolished biofilm formation, while inactivation of the flagellar gene flaA, two component response regulator agrA and the autolysin-adhesin gene ami lead to severe impairment of initial attachment and the subsequent development of a mature biofilm by L. monocytogenes. Mutants in the global regulator of virulence prfA and the alternative sigma factor sigB were unaffected and formed biofilms similar to wild type L. monocytogenes
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| Language |
en
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| Publisher |
Springer
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| Rights |
An edited version of this paper was published by Springer. This paper is for R & D pupose and Copyright [2009] Springer.
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| Subject |
laboratory culture
Listeria monocytogenes biofilms microorganisms |
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| Title |
A study on the effects of some laboratory-derived genetic mutations on biofilm formation by Listeria monocytogenes
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| Type |
Journal Article
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