Densitometric HPTLC Analysis of Juglone, Quercetin, Myricetin, Rutin, Caffeic Acid, and Gallic Acid in Juglans regia L.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Densitometric HPTLC Analysis of Juglone, Quercetin,
Myricetin, Rutin, Caffeic Acid, and Gallic Acid
in Juglans regia L.
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| Creator |
Ghosh, P
Katiyar, Arti |
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| Subject |
Plant sciences
Natural Product Chemistry |
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| Description |
Asensitive, selective, and precise high-performance thin-layer chromatography
(HPTLC) method was developed for simultaneous
analysis of six bioactive phenolic compounds, i.e., juglone,
quercetin, myricetin, rutin, caffeic acid, and gallic acid in methanol
extract and its fractions (hexane, chloroform, ethyl acetate, and
butanol fractions) from bark of Juglans regia. Good separation was
achieved on RP-18 F254S TLC plate using methanol–water–formic
acid–acetic acid (48.8:46.4:2.4:2.4, v/v). The densitometric determination
of the compounds was carried out at 254 nm in reflectance/
absorbance mode. The method was validated in terms of linearity,
sensitivity, accuracy, precision, robustness, and specificity. The linear
regression data for the calibration plots of the reference compounds
showed a good linear relationship with higher correlation
coefficient (r2 ≥ 0.997). Accuracy of the method was evaluated in
terms of average percent recovery, which ranged from 98.63 to
101.06%. HPTLC results revealed qualitative and quantitative differences
in the phenolic compounds in the extract and fractions. The
ethyl acetate fraction contained gallic acid followed by myricetin,
rutin, quercetin, and caffeic acid in higher amount in comparison to
the extract and fractions. The present method can be used for routine
quality control of J. regia extracts.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1281/1/60_Densitometric_Partha_Ghosh.pdf
Ghosh, P and Katiyar, Arti (2012) Densitometric HPTLC Analysis of Juglone, Quercetin, Myricetin, Rutin, Caffeic Acid, and Gallic Acid in Juglans regia L. Journal of Plant Chromatography, 25 (5). pp. 420-425. |
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| Relation |
http://ihbt.csircentral.net/1281/
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