Rapid Validated RP-HPTLC Method for the Quantification of Major Bioactive Constituents of Crataegus oxyacantha L.
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Rapid Validated RP-HPTLC Method for the Quantification
of Major Bioactive Constituents of Crataegus oxyacantha L.
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| Creator |
Kaur, P
Chaudhary, A Katiyar, Arti Singh , Bikram Chand, Gopi Singh, R D |
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| Subject |
Plant sciences
Natural Product Chemistry |
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| Description |
A reverse phase high-performance thin-layer chromatography (RPHPTLC)
method was developed for determination of vitexin, hyperoside,
vitexin-2″-O-rhamnoside, quercetin, and apigenin in the
Crataegus oxyacantha extract. The method employed precoated
plate of RP-18 silica gel 60F254 as the stationary phase with acetonitrile–
methanol–water–formic acid (10:10:20:0.05, v/v) as mobile
phase, and densitometric determination was carried out at wavelength
254 nm in reflection/absorption mode. The linear regression
analysis data for the calibration plots showed linear relationship (r)
from 0.9985 to 0.9992. The method was validated for accuracy, precision,
and robustness. The limits of detection and quantification
were in the range of 100–300 ng and 310–960 ng, respectively, for
analytes. The method is reproducible and convenient for quantitative
analysis of these flavonoids in the leaves of C. oxyacantha. This
is the first report of simultaneous densitometry quantification of
major bioactive constituents in C. oxyacantha by developed and validated
RP-HPTLC method. The change in the content of bioactive
constituents with growth of the plant was also examined in leaf samples
collected for 2 years.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1289/1/67_Rapid_validated_RP%2DHP.pdf
Kaur, P and Chaudhary, A and Katiyar, Arti and Singh , Bikram and Chand, Gopi and Singh, R D (2012) Rapid Validated RP-HPTLC Method for the Quantification of Major Bioactive Constituents of Crataegus oxyacantha L. Journal of Planar Chromatography, 25 (5). pp. 415-419. |
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| Relation |
http://ihbt.csircentral.net/1289/
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