In Vitro Expression and Production of Antibody Against Cymbidium mosaic virus Coat Protein
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
In Vitro Expression and Production of Antibody Against
Cymbidium mosaic virus Coat Protein
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| Creator |
Sherpa, A R
Hallan, Vipin Zaidi , A A |
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| Subject |
Plant sciences
Plant viruses |
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| Description |
Polyclonal rabbit antisera were produced using
coat protein of Cymbidium mosaic virus (CymMV) Indian
isolate expressed in E. coli as GST fusion. The expressed
protein was purified by GST-fusion protein purification kit
for use as an immunogen in rabbits. Antisera prepared in
this manner reacted in double antibody sandwich enzymelinked
immunosorbent assay (DAS ELISA) with extract
from CymMV-infected tissue. The results indicate that
polyclonal antisera prepared from expressed CymMV coat
proteins were useful for the detection of CymMV in an
array of assays. The detection system developed is highly
effective for detection of Indian strain of the virus in
comparison to kits available in the international market.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1295/1/74_In_vitro_Expression.pdf
Sherpa, A R and Hallan, Vipin and Zaidi , A A (2012) In Vitro Expression and Production of Antibody Against Cymbidium mosaic virus Coat Protein. Indian Journal of Virology, 23 (1). pp. 46-49. |
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| Relation |
http://ihbt.csircentral.net/1295/
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