The DNA Adenine Methyltransferase-Encoding Gene (dam) of Vibrio cholerae
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
The DNA Adenine Methyltransferase-Encoding Gene (dam) of
Vibrio cholerae
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| Creator |
Bandyopadhyay, Rupa
Das, Jyotirmoy |
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| Subject |
Infectious Diseases and Immunology
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| Description |
The DNA adenine methyltransferase (MTase)-encoding gene (dam) of Vibrio cholerae, an organism belonging to the
family Vibrionaceae, has been cloned and the complete nucleotide (nt) sequence determined. I’. cholerae dam encodes a
21.5kDa protein and is directly involved in methyl-directed DNA mismatch repair. It can substitute for the Escherichia
coli enzyme and can suppress the phenotypic traits associated with E. coli dam mutants. Overproduction of I’. cholerae
Dam MTase does not result in hypermutability in either I/. cholerae or E. coli cells. Overproduction of I/. cholerae Dam
in a pUC plasmid, however, fails to suppress the 2-aminopurine (2-AP)-sensitive phenotype of E. coli dam mutants.
Homology between the nt and deduced amino acid (aa) sequences of the E. coli and I/ cholerae dam genes is only
30-35%.
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| Date |
1994
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1768/1/GENE__140__(_1)_67%2D71;1994[56].pdf
Bandyopadhyay, Rupa and Das, Jyotirmoy (1994) The DNA Adenine Methyltransferase-Encoding Gene (dam) of Vibrio cholerae. Gene, 140 (1). pp. 67-71. |
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| Relation |
http://dx.doi.org/10.1016/0378-1119(94)90732-3
http://www.eprints.iicb.res.in/1768/ |
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