Recombinant Derivative of a Naturally Occurring Non-Toxinogenic Vibrio cholerae Ol Expressing the B Subunit of Cholera Toxin: a Potential Oral Vaccine Strain
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Recombinant Derivative of a Naturally Occurring Non-Toxinogenic Vibrio cholerae Ol Expressing the B Subunit of Cholera Toxin: a Potential Oral Vaccine Strain
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| Creator |
Dasgupta, Ujjala
Bhadra, Rupak K Panda, Dibyendu K Deb, Amitabha Das, Jyotirmoy Das |
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| Subject |
Infectious Diseases and Immunology
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| Description |
A clinical isolate of Vibrio cholerae O1 was identified which did not possess the heat-labile
( CT), the heat-stable (ST) or the zonula occludens ( Zot) toxin genes. Rabbit ileal loop
assays showed that no other CT-like toxin was produced by this strain. The partly deleted
cholera toxin gene which carries the intact gene for the B subunit was cloned and the
recombinant plasmid, pURD110, was introduced into this non-toxinogenic natural human
isolate. The transformed cells (strain URD2) secreted the B subunit gene product which
competed with the holotoxin secreted by the hypertoxinogenic strain 569B of V. cholerae
for the GM1 ganglioside binding sites in vivo. This strain can colonize the rabbit intestine
as detected by the removable intestinal tie adult rabbit diarrhoea ( RITARD ) model. This
construct has an advantage over other live oral attenuated V. cholerae strains used as
vaccines in that the latter strains were made non-toxinogenic by only deleting part of the
gene coding for the A subunit of cholera toxin while the strain described here is naturally
non-toxinogenic.
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| Publisher |
Elsevier
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| Date |
1994
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1782/1/VACCINE___12__(4)_359%2D364_;1004[63].pdf
Dasgupta, Ujjala and Bhadra, Rupak K and Panda, Dibyendu K and Deb, Amitabha and Das, Jyotirmoy Das (1994) Recombinant Derivative of a Naturally Occurring Non-Toxinogenic Vibrio cholerae Ol Expressing the B Subunit of Cholera Toxin: a Potential Oral Vaccine Strain. Vaccine, 12 (4). pp. 359-364. |
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| Relation |
http://dx.doi.org/10.1016/0264-410X(94)90101-5
http://www.eprints.iicb.res.in/1782/ |
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