Secretion of @Glucosidase by Termitomyces clypeatus: Regulation by Carbon Catabolite Products
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Secretion of @Glucosidase by Termitomyces clypeatus: Regulation by Carbon Catabolite Products
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| Creator |
Khowala, Suman
Sengupta, Subhabrata |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Casamino acids, irrespective of’ carbon source used, highly stimulated extracellular production of
/3-glucosidase by T. clypeatus, which produced very low amounts of enzyme in the presence of 1%
Ot~lu) sugur including xylose in minimal grott,th medium. Casamino acids did not affect the rute of
sugar uptake or improve glucose transport by mushroom, but they sign$cantly reduced the intrul
extracellular enzyme ratio by increasing the secretion of the enzyme from the cell pool into the culture
jiltrate. No phosphoenol pyruvate-mediated transport of sugars was detectable in thefungi. FeNI umino
acids utilized as carbon source by the mushroom and Krebs cycle acids (poorly supporting growth)
sholr,ed activities on enzyme production similar to that of casamino acids. Nonmetabolizable glucose
analogue glucosamine also increased extracellular enzyme production. Liberation of enzyme from
unrushed mycelia MUS stimulated by the presence of glutamate in the incubation mixture and was ulso
found to be insensitive to cycloheximide (30 pg ml-‘). Regulation of the excretion of P-glucosidase in
T. clypeatus by glucose catabolic product(s) MYIS indicated.
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| Date |
1992
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1788/1/ENZYME_AND_MICROBIAL_TECHNOLOGY___14___(2)_144%2D149;1992[54].pdf
Khowala, Suman and Sengupta, Subhabrata (1992) Secretion of @Glucosidase by Termitomyces clypeatus: Regulation by Carbon Catabolite Products. Enzyme and Microbial Technology, 14 (2). pp. 144-149. |
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| Relation |
http://dx.doi.org/10.1016/0141-0229(92)90173-L
http://www.eprints.iicb.res.in/1788/ |
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