Leishmania donovani: Purification and Partial Characterization of a Glycophosphosphingolipid Antigen Expressed on Promastigote Surface
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Leishmania donovani: Purification and Partial Characterization of a Glycophosphosphingolipid Antigen Expressed on Promastigote Surface
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| Creator |
De Majumder, Tripti
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| Subject |
Infectious Diseases and Immunology
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| Description |
Leishmania donovani: Purification and partial characterization of a
glycophosphosphingolipid antigen expressed on promastigote surface. Experimental Parasitology
74,251-260. A ceramide-anchored glycophosphosphingolipid antigen was isolated from the
lipid extract of Leishmania donovani promastigotes. The aflinity-purified glycolipid antigen
contained galactose, mannose, myo-inositol, phosphate, ceramide, and hexosamine but no sialic
acid. The phosphate group was present internally at the core of the structure: inositol (l-O)-
phosphorylceramide. The phosphate group became susceptible to alkaline phosphatase only
after alkali-catalyzed hydrolysis of the glycolipid. o 1s~ Academic press, IIIC.
INDEX DESCRIPTORS AND ABBREVIATIONS: Leishmania donovani; Kala-azar; glycophosphosphingolipid;
membrane antigen; ceramide anchor; GIPL, glycophosphatidylinositol lipids;
GSPL, glycophosphosphingolipid of Leishmania donovani; RIA, radioimmunoassay;
RCA-I, Ricinus communis agglutinin; PMSF, phenylmethylsulfonyl fluoride; H:DE, hexane:
diethyl ether; C:M, chloroform:methanol; GlcNH,, glucosamine; GLC, gas-liquid chromatography;
TFA, trifluoroacetic acid; PBS, phosphate-buffered saline; Solvent A, chloroform:
methanol:ethyl acetate:pyridine:4.5 N ammonia:water (15:15:5:0.5:0.5:0.5, v/v); Solvent
B, acetone:diethyl ether:acetic acid (30:70:2, v/v); Solvent C, chloroform:
methanol:0.25 N ammonium hydroxide in 0.25% KC1 (65:45:9, v/v); Solvent D, 0.01 M
phosphate buffer, pH 6.4, containing 0.05 N ammonium hydroxide and 0.1% sodium salt of
taurodeoxycholic acid; Solvent E, pyridine:ethyl acetate:acetic acid:0.25% KC1 (36:36:7:21,
v/v); Solvent F, I-butanol:pyridine:0.25% KC1 (3:2:1, v/v).
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| Publisher |
Elsevier
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| Date |
1992
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1790/1/EXPERIMENTAL_PARASITOLOGY___74(_3)_251%2D260;1992[36].pdf
De Majumder, Tripti (1992) Leishmania donovani: Purification and Partial Characterization of a Glycophosphosphingolipid Antigen Expressed on Promastigote Surface. Experimental Parasitology, 74 (3). pp. 251-260. |
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| Relation |
http://dx.doi.org/10.1016/0014-4894(92)90148-4
http://www.eprints.iicb.res.in/1790/ |
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