Sandwich Immunoassay of Small Molecules II. Effects of Heterology and Development of a Highly Sensitive and Specific Enzyme Immunoassay for Testosterone
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Sandwich Immunoassay of Small Molecules
II. Effects of Heterology and Development of a Highly Sensitive and Specific
Enzyme Immunoassay for Testosterone
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| Creator |
Sengupta, Judhajit
Dhar, Tarun K Ali, Esahak |
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| Subject |
Infectious Diseases and Immunology
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| Description |
The effect of dimer heterology in the sandwich immunoassay of testosterone was studied using
symmetrical and asymmetrical dimers prepared from testosterone-3-(O-carboxymethyl)oxime and 4-
(carboxymethyl-mercapto)testosterone. The effect of antibody heterology was studied using antibodies
against 3-carboxymethyl oxime and 17-hemisuccinate derivatives of the steroid and an asymmetrical
dimer prepared from the same two derivatives. Using antibody against the 3-carboxymethyl oxime and
the dimer of 4-(carboxymethylmercapto)testosterone, the sensitivity of direct enzyme immunoassay of
testosterone in serum by this method was 0.5 pg/well and the cross-reactivity of 5a-dihydrotestosterone
was 5%.
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| Publisher |
Elsevier
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| Date |
1992
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1793/1/JOURNAL_OF_IMMUNOLOGICAL_METHODS__147_(_2)_181%2D188_;1992[50].pdf
Sengupta, Judhajit and Dhar, Tarun K and Ali, Esahak (1992) Sandwich Immunoassay of Small Molecules II. Effects of Heterology and Development of a Highly Sensitive and Specific Enzyme Immunoassay for Testosterone. Journal of Immunological Methods, 142 (2). pp. 181-188. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/1793/ |
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