Sandwich Immunoassay of Small Molecules I. Investigation with Tstosterone as Model Hapten
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Sandwich Immunoassay of Small Molecules I. Investigation with Tstosterone as Model Hapten
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| Creator |
Ali, Esahak
Sengupta, Judhajit Dhar, Tarun K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
A new strategy has been developed for enzyme immunoassay of small molecules. The method is based
on the formation and estimation of the complex Ab.H-H.Ab* where Ab and Ab* are immobilised and
labeled antibodies respectively and H-H is a synthetic bis-analogue of the hapten H. The amount of the
complex formed is inversely related to the concentration of free hapten present. The estimation of the
sandwich complex increases the sensitivity and the specificity of the assay compared to conventional
assays. Using the N,N'-diamide of testosterone-3-(O-carboxymethyl)oxime with ethylenediamine and a
polyclonal rabbit anti-testosterone antibody (for both labeling and immobilisation) a sensitivity of 100
fg/well (3.5 x 10- 16 mol) was obtained for testosterone. The cross-reactivity of 5a-dihydrotestosterone
was 16% compared to 27% obtained by conventional competitive assays
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| Date |
1992
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1794/1/JOURNAL_OF_IMMUNOLOGICAL_METHODS__147(_2_)173%2D179;1992[49].pdf
Ali, Esahak and Sengupta, Judhajit and Dhar, Tarun K (1992) Sandwich Immunoassay of Small Molecules I. Investigation with Tstosterone as Model Hapten. Journal of Immunological Methods, 147 (2). pp. 173-179. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/1794/ |
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