Isolation and Characterization of a Cyclic Nucleotide-Independent Protein Kinase from Leishmania Donovani
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Isolation and Characterization of a Cyclic Nucleotide-Independent Protein Kinase from Leishmania Donovani
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| Creator |
Banerjee, Chandana
Sarkar, Dwijen |
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| Subject |
Infectious Diseases and Immunology
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| Description |
We describe here a protein kinase from the promastigote form of the parasitic protozoan, Leishmania donovani, purified to near homogeneity to a single-subunit, 34-kDa protein. This enzyme does not require a cofactor, and has several characteristics
in common with the catalytic subunit of mammalian cAMP-dependent protein kinase, for example, preference for kemptide as a substrate, phosphorylation of serine residues of protamine and inhibition by the mammalian heat-stable inhibitor. The leishmanial enzyme can associate with the regulatory subunit of mammalian cAMP-dependent protein kinase to form an inactive holoenzyme that is activated by cAMP and is protected from inhibition by thiol reagents. From these results it is concluded that L. donovani promastigotes possess a protein kinase which has similar characteristics with the mammalian catalytic subunit of cAMP-dependent protein kinase
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| Publisher |
Elsevier
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| Date |
1992
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1797/1/MOLECULAR_AND_BIOCHEMICAL_PARASITOLOGY___52_(_2)195%2D205;1992[33].pdf
Banerjee, Chandana and Sarkar, Dwijen (1992) Isolation and Characterization of a Cyclic Nucleotide-Independent Protein Kinase from Leishmania Donovani. Molecular and Biochemical Parasitology, 52 (2). pp. 195-205. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/1797/ |
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