Thermodynamic Investigations of Ligand–Protein Interactions: Binding of the Phenazinium Dyes Phenosafranin and Safranin O with Human Serum Albumin
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Thermodynamic Investigations of Ligand–Protein Interactions: Binding
of the Phenazinium Dyes Phenosafranin and Safranin O with Human Serum Albumin
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| Creator |
Saha, Ishita
Bhattacharyya, Jhimli Kumar, G Suresh |
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| Subject |
Chemistry
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| Description |
The binding of the phenazinium dyes, phenosafranin (PSF) and safranin O (SO) with human serum albumin was investigated by calorimetry and spectroscopic techniques. Binding parameters revealed that PSF has a higher affinity (K = 1.60 � 105 M�1) compared to SO (K = 0.97 � 105 M�1). The binding of both dyeswas favoured by negative enthalpy and a stronger favourable entropy contribution. The heat capacity values were similar indicating the involvement of similar hydrophobic forces in the complexation. Enthalpyentropy compensation was also observed for both dyes. Both polyelectrolytic and non-polyelectrolytic forces contributed towards the binding but the later was dominant. The fluorescence data suggested a static quenching mechanism. Forster resonance energy transfer studies showed that the specific binding distances between Trp (donor) residue of the protein and the dye (acceptor) molecules were 3.95 and 4.07 nm, respectively, for PSF and SO. Both dyes bind to same site, viz. site I (subdomain II A) of HSA but SO having bulkier groups binds weakly due to steric hindrance
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1812/1/JOURNAL_OF_CHEMICAL_THERMODYNAMICS__56__114%2D122;2013[26].pdf
Saha, Ishita and Bhattacharyya, Jhimli and Kumar, G Suresh (2013) Thermodynamic Investigations of Ligand–Protein Interactions: Binding of the Phenazinium Dyes Phenosafranin and Safranin O with Human Serum Albumin. Journal of Chemical Thermodynamics, 56. pp. 114-122. |
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| Relation |
http://dx.doi.org//10.1016/j.jct.2012.07.010
http://www.eprints.iicb.res.in/1812/ |
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