Validation of a Simple Resazurin-Based Promastigote Assay for the Routine Monitoring of Miltefosine Susceptibility in Clinical Isolates of Leishmania Donovani
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Validation of a Simple Resazurin-Based Promastigote Assay for the Routine Monitoring of Miltefosine Susceptibility in Clinical Isolates of Leishmania Donovani
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| Creator |
Kulshrestha, Arpita
Bhandari, Vasundhra Mukhopadhyay, Rupkatha Ramesh , V Sundar, Shyam Maes , Louis Dujardin , Claude Roy, Syamal Salotra, Poonam |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Simple, cost-effective approach for routine surveillance
of parasite susceptibility to antileishmanial drug
miltefosine (MIL) is highly desirable for controlling emergence
of drug resistance in visceral leishmaniasis (VL). We
validated a simple resazurin-based fluorimetric assay using
promastigotes to track natural MIL tolerance in Leishmania
donovani parasites from VL cases (n017) against standard
amastigote assay, in two different labs in India. The interstage
MIL susceptibility correlated strongly (r00.70, p0
0.0018) using J774.A.1 macrophage cell line-based amastigote
assay and fluorescence-based resazurin assay for promastigotes.
Investigation of inter-stage MIL susceptibility
for the same set of clinical isolates in another lab also
showed a strong correlation (r00.72, p00.0012) using
mouse peritoneal macrophages for amastigote assay and
resazurin-based alamar blue assay for promastigotes. Additionally,
parasites from post-kala-azar dermal leishmaniasis
(PKDL) lesions (n07, r00.78, p00.046) and MIL-induced
parasites (r00.92, p00.0001; n03) also exhibited a strongly
correlated inter-stage miltefosine susceptibility. Thus, our
results support the utility of resazurin assay as a simplified
biological tool for MIL susceptibility monitoring in clinical
isolates from MIL-treated VL/PKDL patients
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| Publisher |
Springer Verlag
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1819/1/PARASITOLOGY_RESEARCH___112(2)_825%2D828_;2013[3].pdf
Kulshrestha, Arpita and Bhandari, Vasundhra and Mukhopadhyay, Rupkatha and Ramesh , V and Sundar, Shyam and Maes , Louis and Dujardin , Claude and Roy, Syamal and Salotra, Poonam (2013) Validation of a Simple Resazurin-Based Promastigote Assay for the Routine Monitoring of Miltefosine Susceptibility in Clinical Isolates of Leishmania Donovani. Parasitology Research, 112 (2). pp. 825-828. |
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| Relation |
http://dx.doi.org/10.1007/s00436-012-3212-3
http://www.eprints.iicb.res.in/1819/ |
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