Organization and Chromosomal Localization of ß-Tubulin Genes in Leishmania Donovani
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Organization and Chromosomal Localization of ß-Tubulin Genes in
Leishmania Donovani
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| Creator |
Das, Saumitra
Adhya, Samit |
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| Subject |
Molecular & Human Genetics
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| Description |
The genomic organization and chromosomal location of the ß-tubulin isogenes
in Leishmania donovani promastigotes has been studied by nucleic acid hybridization techniques using a cloned ß-tubulin gene. We have cloned a ß-tubulin gene fragment, 3·3 kbp long, from genomic DNA of Leishmania donovani using a heterologous ß-tubulin DNA as probe. Restriction maps of this clone have been prepared. It has been estimated that there are approximately 11-15 copies of the ß-tubulin genes per haploid genome. The majority of these isogenes are arranged in a tandem repeat with a length of 3·5 kbp on a single chromosome. In addition a few dispersed gene copies at different chromosomal loci were detected by pulse field gradient gel electrophoresis. Part of the internal coding region of the gene has been sequenced to confirm the identity of the ß-tubulin clone and is found to be nearly identical to that of Leishmania mexicana amazonensis.
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| Publisher |
Cambridge University Press
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| Date |
1990
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1825/1/JOURNAL_OF_BIOSCIENCES___15_(_4)_239%2D248;1990[3].pdf
Das, Saumitra and Adhya, Samit (1990) Organization and Chromosomal Localization of ß-Tubulin Genes in Leishmania Donovani. Journal of BioSciences, 15 (4). pp. 239-248. |
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| Relation |
http://dx.doi.org/10.1007/BF02702665
http://www.eprints.iicb.res.in/1825/ |
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