The Polyclonal Activation of Lymphocytes and T Cell Mitogenicity by a Unique Sialic-Acid-Binding Lectin from the Hemolymph of Achatina Fulica Snail
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
The Polyclonal Activation of Lymphocytes and T Cell Mitogenicity by a Unique Sialic-Acid-Binding Lectin from the Hemolymph of Achatina Fulica Snail
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| Creator |
Mandal, Chitra
Chowdhury, Mridula |
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| Subject |
Infectious Diseases and Immunology
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| Description |
A unique sialic-acid-binding lectin, AchatininH, isolated from the hemolymph of Achatinafulica snail was found to
be strongly mitogenic, as monitored by [3H]thymidine incorporation assay, to rat and human lymphocytes. However, the degree
of mitogenic response varied with the type of cell population. As indicated, this lectin induced proliferation of purified T
lymphocytes and rat thymocytes, whereas it was less mitogenic towards peripheral lymphocytes of pregnant rats and was not
mitogenic towards B lymphocytes. Furthermore, the mitogenic response was inhibited by the sialic-acid-containing disaccharide,
a strong inhibitor of this lectin. This suggests that lymphocyte cell surface molecules containing sialic acid residues are important
for this interaction and may have a structure similar to that of Achatinin H receptors. Although this lectin showed strong
mitogenicity towards lymphocytes, it showed very weak leucoagglutination. Surprisingly, PHA-induced blastogenesis was inhibited by the same dose of Achatininn as caused mitogenic activity in resting lymphocyte culture. The degree of suppression was higher for the lymphocytes isolated from pregnant rat blood compared to the non-pregnant control. Since the 5~Cr-uptake assay and the cell viability test results negate the potential cytotoxic activity of this protein, the immunosuppression induced in the presence of PHA by AchatininH may be the additive effect of two mitogens causing an increased cell-density-dependent arrest. AchatininH does not have complement-like activity, but induces potent polyclonal activation of B cells as measured by the reverse hemolytic plaque assay
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| Date |
1990
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1827/1/JOURNAL_OF_THE_CHEMICAL_SOCIETY%2DCHEMICAL_COMMUNICATIONS__(_21)_1508%2D1509;1990[9].pdf
Mandal, Chitra and Chowdhury, Mridula (1990) The Polyclonal Activation of Lymphocytes and T Cell Mitogenicity by a Unique Sialic-Acid-Binding Lectin from the Hemolymph of Achatina Fulica Snail. Immunopharmacology, 20 (2). pp. 63-72. |
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| Relation |
http://dx.doi.org/10.1016/0162-3109(90)90008-3
http://www.eprints.iicb.res.in/1827/ |
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