Transcription and Processing of ß-Tubulin Messenger RNA in Leishmania Donovani Promastigotes
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Transcription and Processing of ß-Tubulin Messenger RNA in
Leishmania Donovani Promastigotes
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| Creator |
Adhya, Samit
Das, Saumitra Bhaumik, Mantu |
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| Subject |
Molecular & Human Genetics
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| Description |
Transcription of the multicopy ß-tubulin locus in Leishmania donovani
promastigotes was examined by nucleic acid hybridization techniques. By northern analysis of promastigote RNA multiple ß-tubulin mRNAs were detected. The major species of 2·2 kb RNA is derived from the tandem repeat cluster of ß-tubulin genes, the other two (2·4 and 2·6 kb) are presumably derived from dispersed genomic loci. Combined S1-nuclease and primer extension mapping experiments demonstrated the presence of a single 5'-terminus with a 35 nucleotide spliced-leader sequence. The 3'-termini are heterogeneous. The development of a nuclear run-on system in Leishmania for studying transcription of individual genes is reported. Active but transient RNA polymerase II activity was observed in this system. Using specific DNA probes for labelled run-on RNA it was shown that ß-tubulin transcription occurs asymmetrically (i.e., on one strand of the DNA template) in an α-amanitin sensitive manner. The significance of these results for the life cycle of the parasite is discussed.
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| Date |
1990
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1829/1/JOURNAL_OF_BIOSCIENCES___15_4_)(_249%2D259;1990[4].pdf
Adhya, Samit and Das, Saumitra and Bhaumik, Mantu (1990) Transcription and Processing of ß-Tubulin Messenger RNA in Leishmania Donovani Promastigotes. Journal of Biosciences, 15 (4). pp. 249-259. |
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| Relation |
http://dx.doi.org/10.1007/BF02702666
http://www.eprints.iicb.res.in/1829/ |
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