Dephosphorylation of Cell-Surface Phosphoproteins of Goat Spermatozoa
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Dephosphorylation of Cell-Surface Phosphoproteins of Goat Spermatozoa
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| Creator |
Barua, Madhabi
Majumder, G C |
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| Subject |
Cell Biology & Physiology
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| Description |
Multiple ecto-phosphoproteins of the goat cauda-epididymal intact spermatozoa
have been shown to undergo dephosphorylation in vitro by endogenous phosphoprotein phosphatase(s) located on the sperm outer surface. The major ecto-phosphoproteins that are dephosphorylated have molecular masses of 27, 40, 70, 116 and 205 kDa. The cell surface dephosphorylation reaction is not dependent on bivalent metal ions. Mg2+ (5 mM), Mn2+ (5 mM), orthovanadate (200 μΜ) and cAMP (5 μΜ) have no effect on this surface reaction whereas it is inhibited nearly 50% by Co2+ or Zn2+ (1 mM). Spermidine (5 mM), or Ca2+ (1mM) inhibited to a small extent (approx. 25%) the cell surface
dephosphorylation of proteins
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| Date |
1990
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1831/1/JOURNAL_OF_BIOSCIENCES__15(_3)_217%2D221;1990[18].pdf
Barua, Madhabi and Majumder, G C (1990) Dephosphorylation of Cell-Surface Phosphoproteins of Goat Spermatozoa. Journal of Biosciences, 15 (3). pp. 217-221. |
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| Relation |
http://dx.doi.org/10.1007/BF02703888
http://www.eprints.iicb.res.in/1831/ |
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