Comparative Analysis of DNA Methylation Polymorphism in Drought Sensitive (HPKC2) and Tolerant (HPK4) Genotypes of Horse Gram (Macrotyloma uniflorum)
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Comparative Analysis of DNA Methylation
Polymorphism in Drought Sensitive (HPKC2)
and Tolerant (HPK4) Genotypes of Horse Gram
(Macrotyloma uniflorum)
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| Creator |
Bhardwaj, Jyoti
Mahajan, Monika Yadav , S K |
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| Subject |
Nanotechnology
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| Description |
DNA methylation is known as an epigenetic modification that affects
gene expression in plants. Variation in CpG methylation behavior was studied in
two natural horse gram (Macrotyloma uniflorum [Lam.] Verdc.) genotypes, HPKC2
(drought-sensitive) and HPK4 (drought-tolerant). The methylation pattern in both
genotypes was studied through methylation-sensitive amplified polymorphism. The
results revealed that methylation was higher in HPKC2 (10.1%) than in HPK4
(8.6%). Sequencing demonstrated sequence homology with the DRE binding factor
(cbf1), the POZ/BTB protein, and the Ty1-copia retrotransposon among some of the
polymorphic fragments showing alteration in methylation behavior. Differences in
DNA methylation patterns could explain the differential drought tolerance and the
epigenetic signature of these two horse gram genotypes.
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1312/1/88_Comparative.pdf
Bhardwaj, Jyoti and Mahajan, Monika and Yadav , S K (2013) Comparative Analysis of DNA Methylation Polymorphism in Drought Sensitive (HPKC2) and Tolerant (HPK4) Genotypes of Horse Gram (Macrotyloma uniflorum). journal of Biochemical genetics. |
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| Relation |
http://ihbt.csircentral.net/1312/
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