Use of chitinolytic Bacillus atrophaeus strain S2BC-2 antagonistic to Fusarium spp. for control of rhizome rot of ginger
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Use of chitinolytic Bacillus atrophaeus strain S2BC-2
antagonistic to Fusarium spp. for control of rhizome
rot of ginger
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| Creator |
Thakur , Himankshi
Shanmugam, V Gupta, Samriti |
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| Subject |
Plant sciences
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| Description |
An antagonistic bacillus S2BC-2 isolated from
apple rhizosphere soil was identified to be highly chitinolytic
on chitinase detection agar. Standard bacteriological
tests and sequencing of 16S rRNA, and gyrA and rpoB
genes, indicated a taxonomic affiliation of the strain to
Bacillus atrophaeus. The strain was studied for its ability
to grow and produce chitinase on different substrates.
Bacterial cells grown on chitin-containing media showed
enhanced growth and chitinase production with increased
anti-fungal activity against vascular wilt pathogens.
Extracellular proteins of cell-free extracts of media amended
with chitin and fungal cell wall contained 4–10 novel polypeptides.
In polyhouse (bamboo structures that provide protective
shade made of polyvinyl sheet) studies, a chitinsupplemented
talc-based formulation of the S2BC-2 challenge
inoculated with Fusarium oxysporum f. sp. zingiberi
recorded low percent disease indices of 84.9 % and 79.2 %
for yellows and rhizome rot, respectively, over the nonbacterised
pathogen control. The low disease incidences
correlated with 113.3 % maximum rhizome production and
2-fold higher chitinase induction over the pathogen control.
In native gel activity assays, upon challenge-inoculation,
S2BC-2 expressed more chitinase isoforms than the pathogen
control. The results suggest that chitinolytic B. atrophaeus
can be used in the biocontrol of rhizome rot of
ginger.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1334/1/126_Use_of.pdf
Thakur , Himankshi and Shanmugam, V and Gupta, Samriti (2012) Use of chitinolytic Bacillus atrophaeus strain S2BC-2 antagonistic to Fusarium spp. for control of rhizome rot of ginger. Journal of Annals of Microbiology. |
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| Relation |
http://ihbt.csircentral.net/1334/
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