Endogenous auxin level is a critical determinant for in vitro adventitious shoot regeneration in potato (Solanum tuberosum L.)
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
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| Title |
Endogenous auxin level is a critical determinant
for in vitro adventitious shoot regeneration
in potato (Solanum tuberosum L.)
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| Creator |
Pal, A.K.
Acharya, Karan Ahuja, Paramvir Singh |
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| Subject |
Plant sciences
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| Description |
Endogenous levels of indole-3-acetic acid (IAA)
were determined in the internodal explants of Indian
tetraploid potato cultivars (cvs) viz., Kufri Sutlej (K.Sutlej)
and Kufri Giriraj (K.Giriraj). Seven fold higher level of
endogenous IAA was recorded for cv K.Sutlej over cv K.
Giriraj. As a result, perhaps there was a callusing response
from the cut end of the internodal explants of both the cvs in
the MS basal medium. The extent of callusing was relatively
higher in cv K.Sutlej when compared to that in K.Giriraj. The
callusing response was inhibitory to shoot morphogenesis.
Inclusion of an established anti-auxin, 2,3,5-tri-iodobenzoic
acid (TIBA) in the regeneration medium facilitated a high
frequency adventitious shoot regeneration response with
lower cytokinin levels. Murashige and Skoog (MS) medium
containing TIBA at 2.5 mg l−1 and 0.25 mg l−1 zeatin evoked
a 100% regeneration response (4.5 shoot buds per explant) in
cv K.Sutlej within 20–25 days. However, in cv K.Giriraj,
which had lower levels of endogenous IAA, 80% regeneration
response (1.4 shoot buds per explant) was recorded in
an extended period of 40–45 days on a medium containing
0.5 mg l−1 TIBA and 0.1 mg l−1 zeatin. Although, TIBA and
zeatin induced shoot bud formation, it failed to support
sustained growth of the regenerated shoots in cv K.Giriraj.
Hence, 0.01 mg l−1 α-naphthaleneacetic acid (NAA) with a
relatively higher concentration of zeatin (1.0 mg l−1) were
used for sustained shoot regeneration (3.3 shoot buds per
explant) within 25 days. From our results, it is evident that
there was a difference in the requirement of exogenous zeatin
levels required to induce a regeneration response in two
cultivars of potato and this is attributed to the variable levels
of endogenous IAA.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1340/1/38_Endogenous_auxin.pdf
Pal, A.K. and Acharya, Karan and Ahuja, Paramvir Singh (2012) Endogenous auxin level is a critical determinant for in vitro adventitious shoot regeneration in potato (Solanum tuberosum L.). Journal of Plant Biochemistry and Biotechnology, 21 (2). pp. 205-212. |
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| Relation |
http://ihbt.csircentral.net/1340/
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