Antioxidant activity and identification of bioactive compounds from leaves of Anthocephalus cadamba by ultra-performance liquid chromatography/electrospray ionization quadrupole time of flight mass spectrometry
IR@IHBT: CSIR-Institute of Himalayan Bioresource Technology, Palampur
View Archive Info| Field | Value | |
| Title |
Antioxidant activity and identification of bioactive compounds
from leaves of Anthocephalus cadamba by ultra-performance liquid
chromatography/electrospray ionization quadrupole time of flight mass
spectrometry
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| Creator |
Chandel, Madhu
Sharma, Upendra Kumar, Neeraj Singh , Bikram |
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| Subject |
Natural Product Chemistry
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| Description |
Objective: To evaluate the antioxidant potential of different extract/fractions of Anthocephalus
cadamba (A. cadamba) (Roxb.) Miq. (Rubiaceae) and study the tentative identification of their
active constituents. Methods: The extract/fractions were screened for antioxidant activity using
various in vitro assays viz. DPPH assay, ABTS assay, superoxide anion radical scavenging assay,
wreadsu cdientge rpmoiwneerd a bssya cy oalnordi mpleatsrmici dm DetNhAod n. icAkni nugl tarsas-apye. rTfoortaml apnhceen oLlCic- ecloenctternots porf aeyx-trqaucat/dfrraucptoiolen-s tfirmacet ioonf sf liogf hAt . mcaadssa mspbeac. trRoemseutlrtys :m Tehteh oedth wyla sa cuesteadte tfor aacntiaolny swe atsh efo aucntdiv teo cboen mstiotsute anctsti voef ferxatcrtaicotn/
(iEn AaAllC t hfrea catsisoany)s w aass c2o1m.2p4a red to other extract/fractions. The IC50 value of ethyl acetate fraction µg/mL, 1.12 ug/mL, 9.68 µg/mL and 57.81 µg/mL in DPPH assay,
AfrBacTtSio ansss aayl,s ore sdhuocwinegd ptohwe epr oatsesnatyia al ntdo spurpoteercotx itdhee spclaavsemnigdi ng assay respectively. All the extract/ hydroxyl radicals generated by DNA (pBR322) against the attack of Fenton’s reagent. The bioactive compounds were identified by UPLC-ESI-QTOF-MS, by comparing the mass and λmax with literature values. Conclusions: The
pthoatet nthtieayl mofa tyh eb ee xutsreafcutl/ ftrhaecrtaiopnesu ttioc sacgaevnetnsg feo rd tirfefearteinngt frraedei craald-icreallast eind dpiaftfheroelongt iscy dstaemmasg ein.dicated
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://ihbt.csircentral.net/1349/1/136_Antioxidant.pdf
Chandel, Madhu and Sharma, Upendra and Kumar, Neeraj and Singh , Bikram (2012) Antioxidant activity and identification of bioactive compounds from leaves of Anthocephalus cadamba by ultra-performance liquid chromatography/electrospray ionization quadrupole time of flight mass spectrometry. Asian Pacific Journal of Tropical Medicine. pp. 977-985. |
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| Relation |
http://ihbt.csircentral.net/1349/
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