IL-15 activated human peripheral blood dendritic cell kill allogeneic and xenogeneic endothelial cells via apoptosis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
IL-15 activated human peripheral blood dendritic cell kill allogeneic and xenogeneic endothelial cells via apoptosis
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| Creator |
Manna, Partha Pratim
Hira, Sumit Kumar Das, Apabrita Ayan Bandyopadhyay, Santu Gupta, Kailash Kumar |
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| Subject |
Infectious Diseases and Immunology
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| Description |
IL-15 is a pleotropic cytokine, which plays an important role in natural killer (NK) cell activity, T cell proliferation,
and T cell cytotoxic activity. Dendritic cells (DCs) are the major antigen presenting cells in the
immune system and presumed to play an important role in immune recognition of allo and xenotransplantation.
We showed that IL-15 activated human peripheral blood DC is cytotoxic to human and porcine
aortic endothelial cells. Unlike DCs, CD14+ monocytes show no cytotoxicity against the endothelial
cells. This cytotoxic potential of IL-15 activated DC against endothelial cells is dose dependent and
increases significantly upon treatment of endothelial cells with inflammatory cytokines like TNF-a or
IFN-c. The cytotoxic potential of IL-15 activated DC is associated with apoptosis of endothelial cells, as
indicated by the increased Annexin V staining, caspase activation and loss of mitochondrial membrane
potential. Further it was observed that DC mediated cytotoxicity against endothelial cell is mediated
via granzyme B possibly secreted by the activated DCs.
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| Publisher |
Elsevier
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1862/1/CYTOKINE___61(_1)_118%2D126;2013[27].pdf
Manna, Partha Pratim and Hira, Sumit Kumar and Das, Apabrita Ayan and Bandyopadhyay, Santu and Gupta, Kailash Kumar (2013) IL-15 activated human peripheral blood dendritic cell kill allogeneic and xenogeneic endothelial cells via apoptosis. Cytokine, 61 (1). pp. 118-126. |
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| Relation |
http://dx.doi.org/10.1016/j.cyto.2012.09.004
http://www.eprints.iicb.res.in/1862/ |
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