In vivo protein trapping produces a functional expression codex of the vertebrate proteome
IR@IGIB: CSIR-Institute of Genomics & Integrative Biology, New Delhi
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Title |
In vivo protein trapping produces a functional
expression codex of the vertebrate proteome
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Creator |
Clark, Karl J
Balciunas, Darius Pogoda, Hans-Martin Ding, Yonghe Westcot, Stephanie E Bedell, Victoria M Greenwood, Tammy M Urban, Mark D Skuster, Kimberly J Petzold, Andrew M Ni, Jun Nielsen, Aubrey L Patowary , Ashok Scaria, Vinod Sivasubbu, Sridhar Xu, Xiaolei Hammerschmidt, Matthias Ekker, Stephen C |
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Subject |
CH1 Chemical Biology
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Description |
We describe a conditional in vivo protein-trap mutagenesis
system that reveals spatiotemporal protein expression dynamics
and can be used to assess gene function in the vertebrate
Danio rerio
. integration of pGBt-rP2.1 (rP2), a gene-breaking
transposon containing a protein trap, efficiently disrupts
gene expression with >97% knockdown of normal transcript
amounts and simultaneously reports protein expression for
each locus. the mutant alleles are revertible in somatic tissues
via cre recombinase or splice-site-blocking morpholinos and
are thus to our knowledge the first systematic conditional
mutant alleles outside the mouse model. We report a collection
of 350 zebrafish lines that include diverse molecular loci. rP2
integrations reveal the complexity of genomic architecture and
gene function in a living organism and can provide information
on protein subcellular localization. the rP2 mutagenesis
system is a step toward a unified ‘codex’ of protein expression
and direct functional annotation of the vertebrate genome.
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Date |
2011-06
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://openaccess.igib.res.in/155/1/Clark%20et%20al%202011%20Nature%20Methods.pdf
Clark, Karl J and Balciunas, Darius and Pogoda, Hans-Martin and Ding, Yonghe and Westcot, Stephanie E and Bedell, Victoria M and Greenwood, Tammy M and Urban, Mark D and Skuster, Kimberly J and Petzold, Andrew M and Ni, Jun and Nielsen, Aubrey L and Patowary , Ashok and Scaria, Vinod and Sivasubbu, Sridhar and Xu, Xiaolei and Hammerschmidt, Matthias and Ekker, Stephen C (2011) In vivo protein trapping produces a functional expression codex of the vertebrate proteome. In vivo protein trapping produces a functional expression codex of the vertebrate proteome, 8 (6). pp. 10-1038. |
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Relation |
http://openaccess.igib.res.in/155/
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