Highly sensitive detection of proteins using voltammetric assay in the presence of silver nanostructures
IR@CECRI: CSIR-Central Electrochemical Research Institute, Karaikudi
View Archive Info| Field | Value | |
| Title |
Highly sensitive detection of proteins using voltammetric assay in the presence
of silver nanostructures
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| Creator |
Bhavanidevi, L.
Sheela, B. Mandal, A.B. |
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| Subject |
Electrodics and Electrocatalysis
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| Description |
A novel voltammetric protein assay has been demonstrated using bovine serum albumin in the presence
of silver nanostructures (AgNs) formed electrochemically on gold substrates modified by self-assembled
monolayer of thioctic acid. It is shown that the prepared AgNs exhibit voltammetric response characteristic
of Ag and shows near Nernstian response at physiological pH. The advantages of the electrochemical
assay compared to conventional protein analysis are the wide linear concentration range (10�4–10�11 g/
mL) and trace level detection limits (50 pg/mL). The result of this electrochemical analysis agreed well
with an independent spectrophotometric method using Bradford reagent, but the detection limit is far
superior using the electrochemical method. Further, the modified electrode exhibits high sensitivity
and long term stability. We invoke a new concept of surface enhanced activity of Ag+ ions at the electrochemical
interface by the negatively charged bovine serum molecules
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| Publisher |
Elsevier B.V.
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| Date |
2012
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://cecri.csircentral.net/3018/1/021-2012.pdf
Bhavanidevi, L. and Sheela, B. and Mandal, A.B. (2012) Highly sensitive detection of proteins using voltammetric assay in the presence of silver nanostructures. Journal of Electroanalytical Chemistry, 665. pp. 20-25. ISSN 1572-6657 |
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| Relation |
http://www.elsevier.com/locate/jelechem
http://cecri.csircentral.net/3018/ |
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