CD4-independent infection of human peripheral blood dendritic cells with isolates of human immunodeficiency virus type 1
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
CD4-independent infection of human peripheral blood dendritic cells with isolates of human immunodeficiency virus type 1
|
|
| Creator |
Chehimi, Jihed
Prakash, Kesh Shanmugam, Vedapuri Collman, Ronald Jackson, Stephanie J Bandyopadhyay, Santu Start, Stuart E |
|
| Subject |
Infectious Diseases and Immunology
|
|
| Description |
Dendritic cells (DC) are members of a distinct family of
bone marrow-derived leukocytes. DC are potent accessory
cells for a number of T cell-mediated immune
responses, including autologous and allogeneic mixed
leukocyte reactions, and mitogen- and antigenstimulated
lymphocyte proliferation. In the present
study, DC purified from human peripheral blood were
inoculated with various strains (IIIB, SF2, WMJ1,
SF162, 89.6 and clone HXB2) of human immunodeficiency
virus type 1 (HIV-1) displaying different
patterns of cellular tropism. Viral replication was
demonstrated by detection of p24 antigen (Ag) intracellularly and in culture supernatants, and by Southern
and Northern blot analyses for the presence of HIV DNA and RNA, respectively, within infected cells. Cellfree
and cell-associated p24 Ag levels rose substantially
when DC were inoculated with strains SF162, 89.6 and
clone HXB2. In contrast, p24 Ag levels rose only
marginally after inoculation of DC with strains IIIB,
SF2 and WMJ1. Purified DC did not express detectable
membrane CD4, although CD4 mRNA was detected by
reverse transcriptase PCR. The presence of anti-CD4
monoclonal antibodies failed to block infection of DC
by any of the HIV strains tested, suggesting the existence
of a CD4-independent alternative pathway of viral entry.
The possibility that DC serve as a reservoir for HIV-1
must be considered.
|
|
| Publisher |
Society for General Microbiology
|
|
| Date |
1993
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/1908/1/J._Gen._Virol%2C_74%2C_1277%2D1285;1993.pdf
Chehimi, Jihed and Prakash, Kesh and Shanmugam, Vedapuri and Collman, Ronald and Jackson, Stephanie J and Bandyopadhyay, Santu and Start, Stuart E (1993) CD4-independent infection of human peripheral blood dendritic cells with isolates of human immunodeficiency virus type 1. Journal of General Virology, 74. pp. 1277-1285. ISSN 0022-1317 |
|
| Relation |
http://www.eprints.iicb.res.in/1908/
|
|