In Vitro Infection of Natural Killer Cells with Different Human Immunodeficiency Virus Type 1 Isolates
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
In Vitro Infection of Natural Killer Cells with Different Human Immunodeficiency Virus Type 1 Isolates
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| Creator |
Chehimi, Jihed
Bandyopadhyay, Santu Prakash, Kesh Perussia, Bice Hassan, Nassef F Kawashima, Hiasshi Campbell, Donald Kornbluth, Jacki Start, Stuart E |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Natural killer (NK) cells are a discrete subset of leukocytes, distinct from T and B lymphocytes. NK cells
mediate spontaneous non-MHC-restricted killing of a wide variety of target cells without prior sensitization and
appear to be involved in initial protection against certain viral infections. Depressed NK cell-mediated
cytotoxicity, one of the many immunological defects observed in AIDS patients, may contribute to secondary
virus infections. Here we report that clonal and purified polyclonal populations of NK cells, which expressed
neither surface CD4 nor CD4 mRNA, were susceptible to infection with various isolates of human
immunodeficiency virus type 1 (HIV-1). Viral replication was demonstrated by detection of p24 antigen
intracellularly and in culture supernatants, by the presence of HIV DNA within infected cells, and by the ability
of supernatants derived from HIV-infected NK cells to infect peripheral blood mononuclear cells or CD4+ cell
lines. Infection of NK cells was not blocked by anti-CD4 or anti-FcyRIII monoclonal antibodies. NK cells from
HIV-infected and uninfected cultures were similar in their ability to lyse three different target cells.
Considerable numbers of cells died in HIV-infected NK cell cultures. These results suggest that loss of NK cells
in AIDS patients is a direct effect of HIV infection but that reduced NK cell function involves another
mechanism. The possibility that NK cells serve as a potential reservoir for HIV-1 must be considered
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| Publisher |
American Society for Microbiology
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| Date |
1991
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1911/1/J._Virology._65%2C_(4);991.pdf
Chehimi, Jihed and Bandyopadhyay, Santu and Prakash, Kesh and Perussia, Bice and Hassan, Nassef F and Kawashima, Hiasshi and Campbell, Donald and Kornbluth, Jacki and Start, Stuart E (1991) In Vitro Infection of Natural Killer Cells with Different Human Immunodeficiency Virus Type 1 Isolates. Journal of Virology, 65 (4). pp. 1812-1822. |
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| Relation |
http://www.eprints.iicb.res.in/1911/
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