Studies on Enzymes of Trehalose Metabolism from Candida Utilis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Studies on Enzymes of Trehalose Metabolism from Candida Utilis
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| Creator |
Lahiri, Sagar
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| Subject |
Drug Development/Diagnostics & Biotechnology
Structural Biology & Bioinformatics |
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| Description |
Trehalose is a non-reducing disaccharide formed by the 1,1 linkage of two D-glucose molecules [Elbein et al., 2003]. The sugar is present in a wide range of organisms, including
bacteria, yeast, fungi, insects, invertebrates and plants where it may serve as a source of energy and carbon [Elbein et al., 2003]. In comparison with most sugars trehalose is far more stable to wide fluctuations of pH and heat and does not easily interact with proteinaceous molecules
[Richards et al., 2002]. The superior stability of trehalose is largely contributed by the glycosidic
bond joining the two glucose units which is not easily hydrolyzed by acid or alkali and is not cleaved by α-glucosidase [Richards et al., 2002]. The molecular formula and weight of trehalose are C12H22O11 and 342.31, respectively. When purified it is usually found in the dihydrate form, which is the typical commercial product. Physical properties that make trehalose unique are its
high degree of optical rotation ([a]D2 +178º) and its melting behavior [Richards et al., 2002].Trehalose first melts at 97ºC. Additional heat drives off the water of crystallization until the material resolidifies at 130ºC, and then the anhydrous trehalose melts at 203ºC. The combination of the molecular structure and physico - chemical properties of trehalose result in a very stable
disaccharide [Birch et al., 1963, Elbein et al., 1974]. Although α,β (neotrehalose) and β,β(isotrehalose) isomers of trehalose have been synthesized, they are rarely found in nature [Birch et al., 1963, Elbein et al., 1974]. The α,α form is the isomer commonly referred as trehalose
(α,α-trehalose, α-d-glucopyranosyl α-d-glucopyranoside, mushroom sugar or mycose) and is widespread throughout the plant and animal kingdoms [Birch et al.,, 1963, Elbein et al., 1974].
Trehalose is one of the most important storage carbohydrates and serves as a
carbohydrate reservoir in organisms like plants, algae, fungi, yeasts, bacteria, insects and other
invertebrates. Interestingly, for all of the species of insects where trehalose is present, it is the
principal sugar (approximately 80–90%) found in the hemolymph and constitute about 20% of
all carbohydrates during specific stages of insect development [Wyatt et al., 1957, Harding et al.,
1923].
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| Date |
2013
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| Type |
Thesis
NonPeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1916/1/Sagar_Thesis_final.pdf
Lahiri, Sagar (2013) Studies on Enzymes of Trehalose Metabolism from Candida Utilis. PhD thesis, Calcutta University. |
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| Relation |
http://www.eprints.iicb.res.in/1916/
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