Cell biology Of Antigen Processing And Presentation In Leishmania Infection And Its Implications In Antileishmanial Vaccine Development
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Cell biology Of Antigen Processing And Presentation
In Leishmania Infection And Its Implications In
Antileishmanial Vaccine Development
|
|
| Creator |
Ghosh, Moumita
|
|
| Subject |
Cell Biology & Physiology
|
|
| Description |
Protozoan parasites belonging to the genus Leishmania exhibit a pronounced tropism for macrophages. In this thesis, we have highlighted aspects of the surface
chemistry and intracellular antigen processing and presentation of Leishmania donovani infected macrophage.
We recall from our previous study that Leishmania donovani infection causes fluidity in the macrophage membrane. We have showed here the fluidity in infected macrophage membranes are due to decreased cholesterol content in the
membranes. Naturally the question arises, which physical parameters of a biological membrane are influenced by cholesterol? Membrane cholesterol regulates membrane protein function and dynamicity. We have seen a decrease in
antigen presenting ability of MHC class II in Leishmania infection. MHC class II molecule being a transmembrane protein may interact with plasma membrane
components which is responsible for its functional activity. Leishmania infection causes depletion of cholesterol from macrophage membrane which in turn affects
the antigen presenting ability of macrophage, although the precise nature of interaction is still unclear. Measurements of dynamics of membrane proteins
provide another window to show the effect of cholesterol depletion in Leishmania infected macrophage. Studying the local diffusion of membrane protein over time
provide information about the dynamic property of the membrane in Leishmania infection. Biochemically, the cholesterol forms rigid liquid ordered structures
within membranes called lipid rafts. The viscous drag of the lipid rafts restricts the mobility of the proteins. The novel technique allowed us to compare the
diffusion of proteins in normal and Leishmania infected macrophage membrane. Leishmania infection increases the mobility of PLC-δ1 (a lipid raft oriented membrane protein) in macrophages. The lateral diffusion of this protein is again restored back to normal with treatment with liposomal delivery of cholesterol.
|
|
| Date |
2013
|
|
| Type |
Thesis
NonPeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/1917/1/FINAL_MOUMITA_GHOSH.pdf
Ghosh, Moumita (2013) Cell biology Of Antigen Processing And Presentation In Leishmania Infection And Its Implications In Antileishmanial Vaccine Development. PhD thesis, Calcutta University. |
|
| Relation |
http://www.eprints.iicb.res.in/1917/
|
|