Chemical properties and NMR spectroscopic identification of certain fungal siderophores
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Baakza, Arefa
Dave, B P Dube, H C |
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| Date |
2013-11-11T11:03:26Z
2013-11-11T11:03:26Z 2005-10 |
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| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/23255 |
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| Description |
880-886
Siderophores of six fungi <i>viz. Aspergillus </i>sp. ABp4, <i>Aureobacidium pullulans, Penicillium oxalicum</i>,<i> P. chrysosporium</i>, <i>Mycotypha africana </i>and <i>Syncephalastrum racemosum </i>were examined for their (1) electrophoretic<i> </i>mobilities to determine the acidic, basic or neutral charge; (2) Fe (III) binding nature <i>viz., </i>mono-, di-, or trihydroxamate; (3)<i> </i>amino acid composition; and (4) NMR (nuclear magnetic resonance) spectroscopy to determine their structure.<i> </i>Electrophoretic mobilities of siderophores of 3 fungi (<i>P. oxalicum, P. chrysosporium, </i>and <i>M. africana</i>)<i> </i>exhibited net basic<i> </i>charge, siderophores of 2 fungi (<i>Aspergillus </i>sp. ABp4 and <i>S</i>. <i>racemosum) </i>were acidic and 1 fungus (<i>A. pullulans) </i>was neutral. Electrophoresis of ferrated siderophore at <i>p</i>H<i> </i>2 and colour of the spots indicated that siderophores of <i>Aspergillus </i>sp. ABp4 and <i>P. oxalicum </i>and <i>A. pullulans </i>were trihydroxamates, whereas siderophore of <i>P. chrysosporium </i>was dihydroxamate. Amino acid composition of siderophores purified by XAD-2 column chromatography, revealed the presence of asparagine, histidine, and proline in <i>Aspergillus </i>sp. ABp4, serine and alanine in <i>P. chrysosporium, </i>and valine in <i>M. africana. </i>The structure of purified siderophores as revealed by NMR spectroscopy identified siderophore of AB – 2670 (<i>A. pullulans</i>) as asperchrome F1, and AB-513 (<i>M. africana</i>)<i> </i>as rhizoferrin. The peak obtained for siderophore AB-5 (<i>Aspergillus </i>sp. ABp4) did not show resemblance to any known siderophore, therefore may be an exception. |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
IJEB Vol.43(10) [October 2005]
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| Subject |
Amino acids
Fungi NMR Siderophores |
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| Title |
Chemical properties and NMR spectroscopic identification of certain fungal siderophores
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| Type |
Article
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