(CTG)<sub>n</sub> Expansion at DMPK locus seen only in muscle tissue: A novel case
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Hasan, Qurratulain
Mohan, Vasavi Ahuja, Y R |
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| Date |
2013-11-19T05:56:55Z
2013-11-19T05:56:55Z 2004-09 |
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| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/23746 |
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| Description |
937-940
Triplet repeat expansion in 3 untranslated region of myotonic dystrophy protein kinase (DMPK) gene has been implicated as causative in myotonic dystrophy (DM). In cases of DM, high levels of somatic in stability have been reported, in which inter-tissue repeat length differences as large as 3000 repeats have been observed. This study highlights the inter-tissue (CTG)<sub>n</sub> expansion variability at the DMPK locus. Molecular analysis of DMPK gene, encompassing the triplet repeat expansion, was carried out in 31 individuals (11 clinically identified DM patients, 20 controls). All controls showed a 2.1 kb band (upto 35 CTG repeats), while four cases exhibited an expansion (>50 repeats ). A novel observation was made in one case, wherein the D A from lymphocytes showed a normal 2.1 kb band while the muscle tissue DNA from the same patient was heterozygous for normal and 4.3 kb band (> 700 repeats). Our results suggested that because inter-tissue variability existed in the (CTG)<sub>n</sub> repeat number at DMPK locus, an attempt should be made to evaluate affected tissue along with blood wherever possible prior to making a final diagnosis. This is important not only for diagnosis and prenatal analysis, but also while providing genetic counseling to families. |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
IJEB Vol.42(09) [September 2004]
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| Subject |
(CTG)n expansion
DMPK locus Muscle tissue |
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| Title |
(CTG)<sub>n</sub> Expansion at DMPK locus seen only in muscle tissue: A novel case
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| Type |
Article
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