Minor Groove Binding of the Food Colorant Carmoisine to DNA: Spectroscopic and Calorimetric Characterization Studies
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Minor Groove Binding of the Food Colorant Carmoisine to DNA:
Spectroscopic and Calorimetric Characterization Studies
|
|
| Creator |
Basu, Anirban
Kumar, Gopinatha Suresh |
|
| Subject |
Chemistry
|
|
| Description |
The interaction of the food additive carmoisine with herring testes DNA was studied by multifaceted biophysical
techniques. Carmoisine exhibited hypochromic effects in absorbance, whereas in fluorescence the intensity enhanced upon complexation with DNA. Energy transfer from the DNA base pairs to carmoisine molecules occurred upon complexation. A groove binding model of interaction was envisaged for carmoisine−DNA complexation from 4′,6-diamidino-2-phenylindole (DAPI) and Hoechst displacement studies. The binding of carmoisine stabilized the DNA structure against thermal denaturation. The binding induced moderate conformational perturbations in the B-form structure of DNA. The binding affinity (104 M−1) values, calculated from absorbance and fluorescence data, and calorimetry titrations were in close agreement with each other. The binding was characterized to be exothermic and favored by small negative enthalpic and large positive entropic contributions. Salt-dependent calorimetric studies revealed that the binding reaction was dominated by nonpolyelectrolytic forces. The negative heat capacity value suggested the role of hydrophobic effect in the interaction
|
|
| Publisher |
American Chemical Society
|
|
| Date |
2014
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/2022/1/JOURNAL_OF_AGRICULTURAL_AND_FOOD_CHEMISTRY__V_62(_1)_317%2D326__;2014(21).pdf
Basu, Anirban and Kumar, Gopinatha Suresh (2014) Minor Groove Binding of the Food Colorant Carmoisine to DNA: Spectroscopic and Calorimetric Characterization Studies. Journal of Agricultural and Food Chemistry, 62 (1). pp. 317-326. |
|
| Relation |
http://dx.doi.org/10.1021/jf404960n
http://www.eprints.iicb.res.in/2022/ |
|