Simultaneous estimation of E- and Z- isomers of guggulsterone in rabbit plasma using liquid chromatography tandem mass spectrometry and its application to pharmacokinetic study
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
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| Creator |
Bhatta, R S
Chhonker, Y S Jain, G K |
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| Date |
2014-07-21T11:36:17Z
2014-07-21T11:36:17Z 2011 |
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| Identifier |
Biomedical Chromatography, 2011, 25(9), 1054-60
http://hdl.handle.net/123456789/1313 |
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| Description |
A sensitive and selective liquid chromatography / tandem mass spectrometric method was developed for simultaneous determination of E- and Z-guggulsterone isomers (antihyperlipidemic drug) in rabbit plasma. Both the isomers were resolved on Symmetry-Shield C18 (5µm, 4.6 X 150 mm) column, using gradient elution comprising mobile phase of methanol, 0.5% v/v formic acid and acetonitrile. With dexamethasone as internal standard, plasma samples were extracted by an automated solid-phase extraction method using C-18 cartridges. Detection was performed by electrospray ionization in multiple reaction monitoring (MRM) in positive mode. Calibration curve was linear over the concentration range of 1.56-200 ng/ml (r2 0.998) for both analytes. The intra-day and inter-day accuracy and precision were within -0.96 to 4.12 (% bias) and 2.73 to 8.00 (% R.S.D.) respectively. The analytes were stable after three freeze-thaw cycles. The method was successfully applied to study steriospecific pharmacokinetics of E- and Z-guggulsterone in NZ rabbit.
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377683 bytes
application/pdf |
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| Language |
en
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| Relation |
CSIR-CDRI Communication No. 7994.
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| Subject |
Guggulsterone
LC-MS/MS Preclinical pharmacokinetic Rabbit plasma |
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| Title |
Simultaneous estimation of E- and Z- isomers of guggulsterone in rabbit plasma using liquid chromatography tandem mass spectrometry and its application to pharmacokinetic study
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| Type |
Article
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