Single-nucleotide polymorphic macrophage cytokine regulation by Mycobacterium tuberculosis and drug treatment
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Singh, A K
Yadav, A B Garg, Rajiv Misra, Amit |
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| Date |
2014-07-31T10:01:28Z
2014-07-31T10:01:28Z 2014 |
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| Identifier |
Pharmacogenomics, 2014,15(4), 497-508
http://hdl.handle.net/123456789/1324 |
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| Description |
Aims: To investigate survival of Mycobacterium tuberculosis in primary macrophages with single nucleotide polymorphisms (SNPs) affecting cytokine secretion, under treatment with drugs in solution or microparticles.
Materials & Methods: Volunteers were typed for tumor necrosis factor (TNF) (-308 G/A), interleukin (IL)-10 (-1082 A/G) and IL-4 (-590 C/T). Monocyte-derived macrophages (MDMs) were infected in vitro. Cytokine secretion and survival of intracellular bacilli were estimated.
Results: IL-10 AG associated with high secretion in uninfected and infected MDMs (P<0.05) and was reduced more effectively by microparticles than drugs, irrespective of genotype (P<0.05). Differences were observed between IL-4 secretion by CC and TT (P<0.1). Bacteria proliferated more in MDMs from volunteers with higher IL-4 (P<0.05). Microparticles showed higher efficacy (P<0.05) than drugs.
Conclusions: IL-4 and IL-10 SNPs affect the ability of macrophages to counter infection with M tuberculosis. Microparticles elicit favourable macrophage cytokines regardless of SNPs.
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251062 bytes
application/pdf |
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| Language |
en
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| Relation |
CSIR-CDRI Communication No.8573
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| Subject |
SNPs
Inhalable Microparticles Isoniazid, Rifabutin Tumor Necrosis Factor Interleukin 10 Interleukin 4 |
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| Title |
Single-nucleotide polymorphic macrophage cytokine regulation by Mycobacterium tuberculosis and drug treatment
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| Type |
Article
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