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A simple robust enzymatic based high throughput screening method for antimicrobial peptides discovery against Escherichia coli.

IR@CDRI: CSIR-Central Drug Research Institute, Lucknow


Field	Value

Creator	Thirumalai, M K Roy, Arpita Sanikommu, Suma Arockiaraj, Jesu Pasupuleti, Mukesh

Date	2014-08-04T07:59:23Z 2014-08-04T07:59:23Z 2014

Identifier	Journal of Peptide Science, 2014, 20(5), 341-8 http://hdl.handle.net/123456789/1336

Description	The indiscriminate usage of antibiotics has created a major problem in the form of antibiotic resistance. Even though new antimicrobial drug discovery programs have been in place from last two decades, still we are unsuccessful in identifying novel molecules that has a potential to become new therapeutic agents for the treatment of microbial infections. A major problem in most screening studies is the requirement of high throughput techniques. Given this, we present here an enzyme based robust method for screening antimicrobial agent’s active against Escherichia coli. This method is based upon the ability of the intracellular innate enzyme to cleave ONPG (non chromogenic) to ONP (chromogenic) upon the membrane damage or disruption. In comparison to the other currently available methods, we believe that our method provides an opportunity for real time monitoring of the antimicrobial agents action by measuring the ONP generation in a user friendly manner. Even though this method can be applied to other strain, our experience shows that one has to be careful especially when the pigments or metabolites present in the bacteria have the same wavelength absorbance.

Format	685371 bytes application/pdf

Language	en

Relation	CSIR-CDRI Communication No. 8605

Subject	Anti-Infective Agents Antibiotics, Antimicrobial Resistance Antimicrobial Peptides -Galactosidase O-Nitrophenyl Β-D-Galactopyranoside O-Nitrophenolate

Title	A simple robust enzymatic based high throughput screening method for antimicrobial peptides discovery against Escherichia coli.

Type	Article