Development of targeted 1, 2-diacyl-sn-glycero-3-phospho-L-serine coated Gelatin nanoparticles loaded with Amphotericin B for improved in vitro and in vivo effect in Leishmaniasis.
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Khatik, Renuka
Dwivedi, Pankaj Khare, Prashant Kansal, Shaswat Dube, Anuradha Mishra, P R Dwivedi, A K |
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| Date |
2014-08-04T11:30:52Z
2014-08-04T11:30:52Z 2014 |
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| Identifier |
Expert Opinion Drug Delivery, 2014, 11(5), 633-46
http://hdl.handle.net/123456789/1339 |
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| Description |
bjective: The principle objective of this study was to develop phosphatidylserine (PS) coated gelatin nanoparticles (GNPs) bearing amphotericin B (AmB) for specific targeting to the macrophages involved in visceral leishmaniasis (VL).
Method: The two step desolvation method has been used for the preparation of GNPs with AmB which was further coated with PS (PS-AmB-GNPs). The targeting potential of it was compared with uncoated AmB loaded GNPs (AmB-GNPs) for in vitro and in vivo macrophage uptake. Results: The results of flow cytometric data revealed enhanced uptake of PS-AmB-GNPs in J774A.1 macrophage cell lines compared with AmB-GNPs. In vivo organ distribution studies in Wistar rats demonstrated a significantly higher extent of accumulation of PS-AmB-GNPs compared with AmB-GNPs in macrophage-rich organs, particularly in liver and spleen. The in vivo anti-leishmanial activity of plain AmB, AmB-GNPs and PS-AmB-GNPs was tested against VL in Leishmania Donovani - infected hamsters. Highly significant antileishmanial activity (P<0.05 compared with AmB-GNPs) was observed with PS-AmB-GNPs, causing 85.3 ± 7.89% inhibition of splenic parasitic burden. AmB-GNPs and plain AmB caused only 71.0 ± 3.87 % and 50.5 ± 5.12 % parasite inhibition, respectively, in Leishmania infected hamsters (P<0.05 for PS-AmB-GNPs versus plain AmB and AmB-GNPs versus plain AmB).
Conclusion: The objective of the preparation was achieved and high accumulation of AmB in Liver and spleen has been found which resulted in enhanced antileishmanial activity
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581666 bytes
application/pdf |
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| Language |
en
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CSIR-CDRI Communication No. 8613
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| Subject |
Gelatin Nanoparticles
Phosphatidylserine J774A.1 Cell Lines Leishmania Donovani Amastigotes |
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| Title |
Development of targeted 1, 2-diacyl-sn-glycero-3-phospho-L-serine coated Gelatin nanoparticles loaded with Amphotericin B for improved in vitro and in vivo effect in Leishmaniasis.
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| Type |
Article
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