Bioactive cembrane derivatives from the Indian Ocean soft coral, Sinularia kavarattiensis.
IR@NIO: CSIR-National Institute Of Oceanography, Goa
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| Creator |
Lillsunde, K.-E.
Festa, C. Adel, H. DeMarino, S. Lombardi, V. Tilvi, S. Nawrot, D.A. Zampella, A. DeSouza, L. DeAuria, M.V. Tammela, P. |
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| Date |
2014-08-06T12:39:50Z
2014-08-06T12:39:50Z 2014 |
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| Identifier |
Marine Drugs, vol.12(7); 2014; 4045-4068.
http://drs.nio.org/drs/handle/2264/4574 |
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| Description |
Marine organisms and their metabolites represent a unique source of potential pharmaceutical substances. In this study, we examined marine-derived substances for their bioactive properties in a cell-based Chikungunya virus (CHIKV) replicon model and for in vitro anti-inflammatory activity. In the screening of a marine sample library, crude extracts from the Indian soft coral, Sinularia kavarattiensis, showed promising activity against the CHIKV replicon. Bioassay-guided chemical fractionation of S. kavarattiensis resulted in the isolation of six known norcembranoids (1–6) and one new compound, named kavaranolide (7). The structures were elucidated on the basis of NMR and MS spectroscopic data. Compounds 1–3 and 5–7 were evaluated for their replicon-inhibiting potential in the CHIKV model by using a luminescence-based detection technique and live cell imaging. Compounds 1 and 2 showed moderate inhibition of the CHIKV replicon, but imaging studies also revealed cytotoxic properties. Moreover, the effects of the isolated compounds on primary microglial cells, an experimental model for neuroinflammation, were evaluated. Compound 2 was shown to modulate the immune response in microglial cells and to possess potential anti-inflammatory properties by dose-dependently reducing the release of pro- and anti-inflammatory cytokines.
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| Language |
en
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| Publisher |
Published by MDPI AG
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| Rights |
© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/).
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| Subject |
Marine organisms
Bioactive properties |
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| Title |
Bioactive cembrane derivatives from the Indian Ocean soft coral, Sinularia kavarattiensis.
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| Type |
Journal Article
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