Proteomic profiling of γ-ECS overexpressed transgenic Nicotiana in response to drought stress
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Proteomic profiling of γ-ECS overexpressed transgenic
Nicotiana in response to drought stress
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| Creator |
Kumar, Deepak
Datta, Riddhi Sinha, Ragini Ghosh, Aparupa Chattopadhyay, Sharmila |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
The contribution of Glutathione (GSH ) in drought stress tolerance is an established fact. However, the proteins
which are directly or indirectly related to the increased level of GSH in response to drought stress are yet to be
known. To explore this, here, transgenic tobacco plants (NtGp11) overexpressing gamma-glutamylcysteine synthetase
(γ-ECS) was tested for tolerance against drought stress. NtGp11 conferred tolerance to drought stress by increased
germination rate, water retention, water recovery, chlorophyll, and proline content compared with wild-type plants.
Semi-quantitative RT-PCR analysis revealed that the transcript levels of stress-responsive genes were higher in
NtGp11 compared with wild-type in response to drought stress. Two-dimensional gel electrophoresis (2-DE) coupled
with MALDI TOF-TOF MS/MS analysis has been used to identify 43 differentially expressed proteins in response to
drought in wild-type and NtGp11 plants. The results demonstrated the up-accumulation of 58.1% of proteins among
which 36%, 24%, and 20% of them were related to stress and defense, carbon metabolism and energy metabolism
categories, respectively. Taken together, our results demonstrated that GSH plays an important role in combating
drought stress in plants by inducing stress related genes and proteins like HSP70, chalcone synthase, glutathione
peroxidase, thioredoxin peroxidase, ACC oxidase, and heme oxygenase I.
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| Publisher |
Landes Bioscience
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| Date |
2014
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2079/1/2015.pdf
Kumar, Deepak and Datta, Riddhi and Sinha, Ragini and Ghosh, Aparupa and Chattopadhyay, Sharmila (2014) Proteomic profiling of γ-ECS overexpressed transgenic Nicotiana in response to drought stress. Plant Signaling & Behavio, 9. ISSN 1559-2316 |
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| Relation |
http://dx.doi.org/10.4161/psb.29246
http://www.eprints.iicb.res.in/2079/ |
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