In-vitro evaluation of Bacopa monniera extract and individual constituents on human recombinant Monoamine oxidase enzymes
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Singh, Rajbir
Ramakrishna, Rachumallu Bhateria, Manisha Bhatta, R S |
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| Date |
2015-02-25T07:38:34Z
2015-02-25T07:38:34Z 2014 |
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| Identifier |
Phytotherapy Research, 2014, 28 (9), 1419–1422
http://hdl.handle.net/123456789/1427 |
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| Description |
Bacopa monniera (BM) is a traditional Ayurvedic medicinal plant that has been used worldwide for its nootropic action. Chemically standardized extract of BM is now available as over the counter herbal remedy to enhance memory in children and adults. Considering the nootropic action of BM, we evaluated the effect of clinically available BM extract and six of BM constituents (bacoside A3, bacopaside I, bacopaside II, bacosaponin C, bacosine and bacoside A mixture) on recombinant human monoamine oxidase enzymes. Effect of BM extract and individual constituents on human recombinant MAO-A and MAO-B enzymes were evaluated using MAO-GloTM assay kit (Promega corporation,USA), following the instructional manual. IC50 and mode of inhibition was measured for MAO enzymes. Bacopaside I and bacoside A mix inhibited the MAO-A and MAO-B enzymes. Bacopaside I exhibited mixed mode of inhibition with IC50 and Ki value of 17.08 ± 1.64 and 42.5 ± 3.53 µg/mL, respectively for MAO-A enzyme. Bacopaside I is the major constituent of BM that inhibited the MAO-A enzyme selectively.
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| Format |
316589 bytes
application/pdf |
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| Language |
en
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| Relation |
CSIR-CDRI Communication No. 8590
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| Subject |
Bacopa monniera
Monoamine oxidase enzymes |
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| Title |
In-vitro evaluation of Bacopa monniera extract and individual constituents on human recombinant Monoamine oxidase enzymes
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| Type |
Article
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