CSIR Central

Bacterial bioluminescence response to long-term exposure to reverse osmosis treated effluents from dye industries

IR@NIO: CSIR-National Institute Of Oceanography, Goa


Field	Value

Creator	Ravindran, J. Manikandan, B. Shirodkar, P.V. Francis, K.X. ManiMurali, R. Vethamony, P.

Date	2014-12-11T06:39:31Z 2014-12-11T06:39:31Z 2014

Identifier	Canadian Journal of Microbiology, vol.60(10); 2014; 661-668 http://drs.nio.org/drs/handle/2264/4654

Description	The bacterial bioluminescence assay is one of the novel means for toxicity detection. The bioluminescence response of 2 marine bioluminescent bacteria was tested upon their long-term exposure to 9 different reverse osmosis (RO) rejects with varying chemical composition sampled from various dye industries. Bioluminescent bacteria were cultured in the RO reject samples, at different concentrations, and their growth rate and luminescence was measured for 24 h. The RO reject samples caused sublethal effects upon exposure and retarded the growth of bacteria, confirming their toxic nature. Further, continuation of the exposure showed that the initial luminescence, though reduced, recovered and increased beyond the control cultures irrespective of cell density, and finally decreased once again. The present study emphasizes the need of evolving a long-term exposure assay and shows that the method followed in this study is suitable to evaluate the toxicants that exert delayed toxicity, using lower concentrations of toxicants as well as coloured samples.

Language	en

Publisher	NRC Research Press

Rights	© NRC Research Press. All authors who publish in NRC Research Press journals may at any time in the publication process self-archive their submitted and/or accepted manuscript on their institution's Web site or in an open-access repository.

Subject	Bioluminescence, Reverse Osmosis lethal effect pollution

Title	Bacterial bioluminescence response to long-term exposure to reverse osmosis treated effluents from dye industries

Type	Journal Article