Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids
IR@NIO: CSIR-National Institute Of Oceanography, Goa
View Archive Info| Field | Value | |
| Creator |
Augustine, M.S.
Anas, A. Das, A.V. Sreekanth, S. Jayalekshmi, S. |
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| Date |
2015-02-17T04:59:45Z
2015-02-17T04:59:45Z 2015 |
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| Identifier |
Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, vol.136; 327–333
http://drs.nio.org/drs/handle/2264/4687 |
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| Description |
Highly luminescent, manganese doped, zinc sulphide (ZnS:Mn) nanocrystals biofunctionalized with chitosan and various aminoacids such as l-citrulline, l-lysine, l-arginine, l-serine, l-histidine and glycine were synthesized by chemical capping co-precipitation method at room temperature, which is a simple and cost effective technique. The synthesized nanocrystals were structurally characterized by TEM, XRD, EDXS and FT-IR spectroscopy techniques. They possess high colloidal stability with strong orange red photoluminescence emission at 598 nm. The intensity of orange red emission has been observed to be maximum in l-citrulline capped ZnS:Mn nanocrystals in which the emission at 420 nm is effectively quenched by surface passivation due to capping. Taking into consideration the prospects of these highly luminescent, bio-compatible ZnS:Mn nanocrystals in bio-imaging applications, cytotoxicity studies were conducted to identify the capping combination which would accomplish minimum toxic effects. ZnS:Mn nanocrystals biofunctionalized with chitosan, l-citrulline, glycine, l-artginine, l-serine and l-histidine showed least toxicity up to 10 nM concentrations in mouse fibroblast L929 cells, which further confirms their cytocompatibility. Also the ZnS:Mn nanocrystals biofunctionalized with l-arginine showed maximum uptake in in vitro studies carried out in human embryonic kidney cells, HEK-293T, which shows the significant role of this particular amino acid in fetoplacental nutrition. The present study highlights the suitability of aminoacid conjugated ZnS:Mn nanocrystals, as promising candidates for biomedical applications
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| Language |
en
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| Publisher |
Elsevier
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| Rights |
An edited version of this paper was published by Elsevier. Copyright [2014] Elsevier
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| Subject |
spectroscopic techniques
toxicity Amino acids nanocrystal |
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| Title |
Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids
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| Type |
Journal Article
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